src 2110 Search Results


mouse monoclonal anti src  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc mouse monoclonal anti src
( A ) MDA-MB-231 and BT-549 cells were transfected with a pool of 4 oligonucleotides (100 nM) that bind and <t>degrade</t> <t>CDCP1</t> mRNA (see Materials and Methods for sequences) or with the appropriate negative control siRNAs. Cells were harvested at 48 h post-transfection, and CDCP1 expression was verified by western blot. Monoclonal anti-actin was used as a loading control. The knockdown efficiency was ≥ 60% for both CDCP1 forms. ( B ) CDCP1 siRNA-treated and control-treated MDA-MB-231 and BT-549 cells were analyzed for activation of CDCP1, <t>Src,</t> and PKCδ. Monoclonal anti-actin and anti-vinculin were used as loading controls.
Mouse Monoclonal Anti Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti src/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
mouse monoclonal anti src - by Bioz Stars, 2026-05
95/100 stars
  Buy from Supplier

Image Search Results


( A ) MDA-MB-231 and BT-549 cells were transfected with a pool of 4 oligonucleotides (100 nM) that bind and degrade CDCP1 mRNA (see Materials and Methods for sequences) or with the appropriate negative control siRNAs. Cells were harvested at 48 h post-transfection, and CDCP1 expression was verified by western blot. Monoclonal anti-actin was used as a loading control. The knockdown efficiency was ≥ 60% for both CDCP1 forms. ( B ) CDCP1 siRNA-treated and control-treated MDA-MB-231 and BT-549 cells were analyzed for activation of CDCP1, Src, and PKCδ. Monoclonal anti-actin and anti-vinculin were used as loading controls.

Journal: Oncotarget

Article Title: CDCP1 is a novel marker of the most aggressive human triple-negative breast cancers

doi: 10.18632/oncotarget.11935

Figure Lengend Snippet: ( A ) MDA-MB-231 and BT-549 cells were transfected with a pool of 4 oligonucleotides (100 nM) that bind and degrade CDCP1 mRNA (see Materials and Methods for sequences) or with the appropriate negative control siRNAs. Cells were harvested at 48 h post-transfection, and CDCP1 expression was verified by western blot. Monoclonal anti-actin was used as a loading control. The knockdown efficiency was ≥ 60% for both CDCP1 forms. ( B ) CDCP1 siRNA-treated and control-treated MDA-MB-231 and BT-549 cells were analyzed for activation of CDCP1, Src, and PKCδ. Monoclonal anti-actin and anti-vinculin were used as loading controls.

Article Snippet: In the biochemical analyses, we used rabbit polyclonal antibody against CDCP1 (Merck Millipore); rabbit polyclonal phospho-CDCP1 (Tyr734) (Cell Signaling); mouse monoclonal anti-Src, clone GD11 (Merck Millipore); rabbit polyclonal phospho-Src family (Tyr416) (Cell Signaling); rabbit polyclonal anti-PKCδ (Cell Signaling); rabbit polyclonal phospho-PKCδ (Tyr311) (Cell Signaling); mouse monoclonal anti-vinculin, clone hVIN-1 (Sigma); anti-rabbit or -mouse IgG (GE Healthcare) as the secondary antibody; and peroxidase-linked mouse monoclonal anti-actin (Sigma-Aldrich).

Techniques: Transfection, Negative Control, Expressing, Western Blot, Activation Assay